RNA

Part:BBa_K4345010:Design

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-29)


RNA thermometer prfA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.


Source

L. monocytogenes virulence modulation, often via cross-talk with PrfA, or by regulating new genes involved in host colonization.

References

Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P. An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell. 2002;110(5):551-561. doi:10.1016/s0092-8674(02)00905-4